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f4 80 percp cy5 5  (Elabscience Biotechnology)
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(A) Schematic illustration of Trem2 antibody–siRNA conjugates used for Plgrkt knockdown in SAMs. (B) Uptake of <t>Cy5-labeled</t> Trem2–siRNA conjugates by RAW264.7 macrophages observed by fluorescence microscopy. (C) In vivo localization of Cy5-labeled Trem2–siRNA conjugates with <t>F4/80-Cy3-positive</t> macrophages in liver tissue. (D) Schematic overview of the BDL mouse model and treatment groups. (E) Western blot analysis of Plg-RKT protein levels in liver tissues from different experimental groups. (F) H&E and Sirius Red staining of liver sections from each group. (G) Representative flow cytometry analysis of SAMs (Cd45 + F4/80 + Trem2 + Cd9 + ) in liver tissue. (H) Quantification of SAM frequencies across experimental groups. (I) mRNA expression levels of SAM-associated genes ( Trem2 and Cd9 ) measured by RT-qPCR. (J) mRNA expression levels of fibrosis-related genes ( Spp1 , Anxa2 , Gpnmb , Lgals1 , and Lgals3 ) measured by RT-qPCR. Data represent n = 5 per group. * P < 0.05, ** P < 0.01, and *** P < 0.001. BDL, bile duct ligation; H&E, hematoxylin and eosin; RT-qPCR, reverse transcription quantitative polymerase chain reaction; SAMs, scar-associated macrophages; siRNA, small interfering RNA; PLGRKT, plasminogen receptor with a C-terminal lysine.
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(A) Schematic illustration of Trem2 antibody–siRNA conjugates used for Plgrkt knockdown in SAMs. (B) Uptake of <t>Cy5-labeled</t> Trem2–siRNA conjugates by RAW264.7 macrophages observed by fluorescence microscopy. (C) In vivo localization of Cy5-labeled Trem2–siRNA conjugates with <t>F4/80-Cy3-positive</t> macrophages in liver tissue. (D) Schematic overview of the BDL mouse model and treatment groups. (E) Western blot analysis of Plg-RKT protein levels in liver tissues from different experimental groups. (F) H&E and Sirius Red staining of liver sections from each group. (G) Representative flow cytometry analysis of SAMs (Cd45 + F4/80 + Trem2 + Cd9 + ) in liver tissue. (H) Quantification of SAM frequencies across experimental groups. (I) mRNA expression levels of SAM-associated genes ( Trem2 and Cd9 ) measured by RT-qPCR. (J) mRNA expression levels of fibrosis-related genes ( Spp1 , Anxa2 , Gpnmb , Lgals1 , and Lgals3 ) measured by RT-qPCR. Data represent n = 5 per group. * P < 0.05, ** P < 0.01, and *** P < 0.001. BDL, bile duct ligation; H&E, hematoxylin and eosin; RT-qPCR, reverse transcription quantitative polymerase chain reaction; SAMs, scar-associated macrophages; siRNA, small interfering RNA; PLGRKT, plasminogen receptor with a C-terminal lysine.
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(A) Schematic illustration of Trem2 antibody–siRNA conjugates used for Plgrkt knockdown in SAMs. (B) Uptake of <t>Cy5-labeled</t> Trem2–siRNA conjugates by RAW264.7 macrophages observed by fluorescence microscopy. (C) In vivo localization of Cy5-labeled Trem2–siRNA conjugates with <t>F4/80-Cy3-positive</t> macrophages in liver tissue. (D) Schematic overview of the BDL mouse model and treatment groups. (E) Western blot analysis of Plg-RKT protein levels in liver tissues from different experimental groups. (F) H&E and Sirius Red staining of liver sections from each group. (G) Representative flow cytometry analysis of SAMs (Cd45 + F4/80 + Trem2 + Cd9 + ) in liver tissue. (H) Quantification of SAM frequencies across experimental groups. (I) mRNA expression levels of SAM-associated genes ( Trem2 and Cd9 ) measured by RT-qPCR. (J) mRNA expression levels of fibrosis-related genes ( Spp1 , Anxa2 , Gpnmb , Lgals1 , and Lgals3 ) measured by RT-qPCR. Data represent n = 5 per group. * P < 0.05, ** P < 0.01, and *** P < 0.001. BDL, bile duct ligation; H&E, hematoxylin and eosin; RT-qPCR, reverse transcription quantitative polymerase chain reaction; SAMs, scar-associated macrophages; siRNA, small interfering RNA; PLGRKT, plasminogen receptor with a C-terminal lysine.
Anti Hla Dr Percp Cy5 5, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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flow cytometry percp cy5 5 anti mouse cd45 antibody  (Elabscience Biotechnology)
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(A) Schematic illustration of Trem2 antibody–siRNA conjugates used for Plgrkt knockdown in SAMs. (B) Uptake of <t>Cy5-labeled</t> Trem2–siRNA conjugates by RAW264.7 macrophages observed by fluorescence microscopy. (C) In vivo localization of Cy5-labeled Trem2–siRNA conjugates with <t>F4/80-Cy3-positive</t> macrophages in liver tissue. (D) Schematic overview of the BDL mouse model and treatment groups. (E) Western blot analysis of Plg-RKT protein levels in liver tissues from different experimental groups. (F) H&E and Sirius Red staining of liver sections from each group. (G) Representative flow cytometry analysis of SAMs (Cd45 + F4/80 + Trem2 + Cd9 + ) in liver tissue. (H) Quantification of SAM frequencies across experimental groups. (I) mRNA expression levels of SAM-associated genes ( Trem2 and Cd9 ) measured by RT-qPCR. (J) mRNA expression levels of fibrosis-related genes ( Spp1 , Anxa2 , Gpnmb , Lgals1 , and Lgals3 ) measured by RT-qPCR. Data represent n = 5 per group. * P < 0.05, ** P < 0.01, and *** P < 0.001. BDL, bile duct ligation; H&E, hematoxylin and eosin; RT-qPCR, reverse transcription quantitative polymerase chain reaction; SAMs, scar-associated macrophages; siRNA, small interfering RNA; PLGRKT, plasminogen receptor with a C-terminal lysine.
Flow Cytometry Percp Cy5 5 Anti Mouse Cd45 Antibody, supplied by Elabscience Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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(A) Schematic illustration of Trem2 antibody–siRNA conjugates used for Plgrkt knockdown in SAMs. (B) Uptake of <t>Cy5-labeled</t> Trem2–siRNA conjugates by RAW264.7 macrophages observed by fluorescence microscopy. (C) In vivo localization of Cy5-labeled Trem2–siRNA conjugates with <t>F4/80-Cy3-positive</t> macrophages in liver tissue. (D) Schematic overview of the BDL mouse model and treatment groups. (E) Western blot analysis of Plg-RKT protein levels in liver tissues from different experimental groups. (F) H&E and Sirius Red staining of liver sections from each group. (G) Representative flow cytometry analysis of SAMs (Cd45 + F4/80 + Trem2 + Cd9 + ) in liver tissue. (H) Quantification of SAM frequencies across experimental groups. (I) mRNA expression levels of SAM-associated genes ( Trem2 and Cd9 ) measured by RT-qPCR. (J) mRNA expression levels of fibrosis-related genes ( Spp1 , Anxa2 , Gpnmb , Lgals1 , and Lgals3 ) measured by RT-qPCR. Data represent n = 5 per group. * P < 0.05, ** P < 0.01, and *** P < 0.001. BDL, bile duct ligation; H&E, hematoxylin and eosin; RT-qPCR, reverse transcription quantitative polymerase chain reaction; SAMs, scar-associated macrophages; siRNA, small interfering RNA; PLGRKT, plasminogen receptor with a C-terminal lysine.
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anti mouse cd8α percp cy5 5 multi sciences 70 f2100804  (Multi Sciences (Lianke) Biotech Co Ltd)
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(A) Schematic illustration of Trem2 antibody–siRNA conjugates used for Plgrkt knockdown in SAMs. (B) Uptake of <t>Cy5-labeled</t> Trem2–siRNA conjugates by RAW264.7 macrophages observed by fluorescence microscopy. (C) In vivo localization of Cy5-labeled Trem2–siRNA conjugates with <t>F4/80-Cy3-positive</t> macrophages in liver tissue. (D) Schematic overview of the BDL mouse model and treatment groups. (E) Western blot analysis of Plg-RKT protein levels in liver tissues from different experimental groups. (F) H&E and Sirius Red staining of liver sections from each group. (G) Representative flow cytometry analysis of SAMs (Cd45 + F4/80 + Trem2 + Cd9 + ) in liver tissue. (H) Quantification of SAM frequencies across experimental groups. (I) mRNA expression levels of SAM-associated genes ( Trem2 and Cd9 ) measured by RT-qPCR. (J) mRNA expression levels of fibrosis-related genes ( Spp1 , Anxa2 , Gpnmb , Lgals1 , and Lgals3 ) measured by RT-qPCR. Data represent n = 5 per group. * P < 0.05, ** P < 0.01, and *** P < 0.001. BDL, bile duct ligation; H&E, hematoxylin and eosin; RT-qPCR, reverse transcription quantitative polymerase chain reaction; SAMs, scar-associated macrophages; siRNA, small interfering RNA; PLGRKT, plasminogen receptor with a C-terminal lysine.
Anti Mouse Cd8α Percp Cy5 5 Multi Sciences 70 F2100804, supplied by Multi Sciences (Lianke) Biotech Co Ltd, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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(A) Schematic illustration of Trem2 antibody–siRNA conjugates used for Plgrkt knockdown in SAMs. (B) Uptake of <t>Cy5-labeled</t> Trem2–siRNA conjugates by RAW264.7 macrophages observed by fluorescence microscopy. (C) In vivo localization of Cy5-labeled Trem2–siRNA conjugates with <t>F4/80-Cy3-positive</t> macrophages in liver tissue. (D) Schematic overview of the BDL mouse model and treatment groups. (E) Western blot analysis of Plg-RKT protein levels in liver tissues from different experimental groups. (F) H&E and Sirius Red staining of liver sections from each group. (G) Representative flow cytometry analysis of SAMs (Cd45 + F4/80 + Trem2 + Cd9 + ) in liver tissue. (H) Quantification of SAM frequencies across experimental groups. (I) mRNA expression levels of SAM-associated genes ( Trem2 and Cd9 ) measured by RT-qPCR. (J) mRNA expression levels of fibrosis-related genes ( Spp1 , Anxa2 , Gpnmb , Lgals1 , and Lgals3 ) measured by RT-qPCR. Data represent n = 5 per group. * P < 0.05, ** P < 0.01, and *** P < 0.001. BDL, bile duct ligation; H&E, hematoxylin and eosin; RT-qPCR, reverse transcription quantitative polymerase chain reaction; SAMs, scar-associated macrophages; siRNA, small interfering RNA; PLGRKT, plasminogen receptor with a C-terminal lysine.
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(A) Schematic illustration of Trem2 antibody–siRNA conjugates used for Plgrkt knockdown in SAMs. (B) Uptake of Cy5-labeled Trem2–siRNA conjugates by RAW264.7 macrophages observed by fluorescence microscopy. (C) In vivo localization of Cy5-labeled Trem2–siRNA conjugates with F4/80-Cy3-positive macrophages in liver tissue. (D) Schematic overview of the BDL mouse model and treatment groups. (E) Western blot analysis of Plg-RKT protein levels in liver tissues from different experimental groups. (F) H&E and Sirius Red staining of liver sections from each group. (G) Representative flow cytometry analysis of SAMs (Cd45 + F4/80 + Trem2 + Cd9 + ) in liver tissue. (H) Quantification of SAM frequencies across experimental groups. (I) mRNA expression levels of SAM-associated genes ( Trem2 and Cd9 ) measured by RT-qPCR. (J) mRNA expression levels of fibrosis-related genes ( Spp1 , Anxa2 , Gpnmb , Lgals1 , and Lgals3 ) measured by RT-qPCR. Data represent n = 5 per group. * P < 0.05, ** P < 0.01, and *** P < 0.001. BDL, bile duct ligation; H&E, hematoxylin and eosin; RT-qPCR, reverse transcription quantitative polymerase chain reaction; SAMs, scar-associated macrophages; siRNA, small interfering RNA; PLGRKT, plasminogen receptor with a C-terminal lysine.

Journal: Journal of Clinical and Translational Hepatology

Article Title: Single-cell RNA Sequencing Analysis Reveals That Targeting PLG–PLGRKT Signaling-mediated Pro-fibrotic Scar-associated Macrophages Ameliorates Liver Fibrosis in Biliary Atresia

doi: 10.14218/JCTH.2026.00278

Figure Lengend Snippet: (A) Schematic illustration of Trem2 antibody–siRNA conjugates used for Plgrkt knockdown in SAMs. (B) Uptake of Cy5-labeled Trem2–siRNA conjugates by RAW264.7 macrophages observed by fluorescence microscopy. (C) In vivo localization of Cy5-labeled Trem2–siRNA conjugates with F4/80-Cy3-positive macrophages in liver tissue. (D) Schematic overview of the BDL mouse model and treatment groups. (E) Western blot analysis of Plg-RKT protein levels in liver tissues from different experimental groups. (F) H&E and Sirius Red staining of liver sections from each group. (G) Representative flow cytometry analysis of SAMs (Cd45 + F4/80 + Trem2 + Cd9 + ) in liver tissue. (H) Quantification of SAM frequencies across experimental groups. (I) mRNA expression levels of SAM-associated genes ( Trem2 and Cd9 ) measured by RT-qPCR. (J) mRNA expression levels of fibrosis-related genes ( Spp1 , Anxa2 , Gpnmb , Lgals1 , and Lgals3 ) measured by RT-qPCR. Data represent n = 5 per group. * P < 0.05, ** P < 0.01, and *** P < 0.001. BDL, bile duct ligation; H&E, hematoxylin and eosin; RT-qPCR, reverse transcription quantitative polymerase chain reaction; SAMs, scar-associated macrophages; siRNA, small interfering RNA; PLGRKT, plasminogen receptor with a C-terminal lysine.

Article Snippet: Non-parenchymal cells (1 × 10 6 cells) were Fc-blocked with TruStain FcX (BioLegend, San Diego, CA, USA) and stained with CD45 PE/Cy7 (BioLegend, 103113), F4/80 PerCP/Cy5.5 (Elabscience, E-AB-F0995UJ; Houston, TX, USA), TREM2 PE (BioLegend, 824805), CD9 APC (BioLegend, 124811), and Ly6G BV421 (BioLegend, 127627)( ).

Techniques: Knockdown, Labeling, Fluorescence, Microscopy, In Vivo, Tissue, Western Blot, Analysis, Staining, Flow Cytometry, Expressing, Quantitative RT-PCR, Ligation, Reverse Transcription, Real-time Polymerase Chain Reaction, Small Interfering RNA